Features

• Produces up to 3 mg/ml protein in under 48 hours.
• Simple reaction set-up – just add DNA
• Capable of catalyzing complex post-translational modifications

Description

The ALiCE® cell-free protein expression kits set a new benchmark for protein production. The kits contain a lysate derived from N. tabacum c.v. BY-2 cells which have been specially optimized to produce consistently high protein yields of up to 3 mg per mL (eYFP). The inclusion of native microsomes derived from the endoplasmic reticulum and Golgi apparatus means the lysate is also capable of producing proteins with complex eukaryotic post-translational modifications, including disulfide bonds and glycosylation. The kits are provided with two vectors, pALiCE01, and pALiCE02 respectively, which enable the user to produce proteins via cytosolic or microsomal pathways. These elements combined, provide a powerful expression tool capable of producing even the most difficult-to-express proteins.