Scalable protein expression

Seamlessly scalable protein production from microliter to liter production

The first fully scalable eukaryotic cell-free expression platform


From screening campaign to characterization and developability assessment-scale protein production in a matter of days – without the need for cell line development or process optimization. ALiCE®, our pioneering scalable eukaryotic cell-free protein synthesis platform, makes this dream a tangible reality, accelerating protein exploration across a spectrum of applications in basic research to biopharma development.

ALiCE® lysate production is on track for GMP compliance

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    Linear scaling of eYFP from microliter to mililiter with ALiCE

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    Linear scaling of glucose oxidase from microliter to mililiter with ALiCE

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    Linear scaling of eYFP from microliter to liter scale with ALiCE

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Challenging the protein expression status quo


For decades, cell-free protein synthesis has been renowned for its ability to rapidly express proteins resistant to production in cell-based systems. Yet, the challenge of scaling eukaryotic systems, essential for producing complex proteins, has hindered widespread adoption and limited scientists leveraging the full power of this technology. ALiCE® is different: Our team of innovators have developed ALiCE® to work as effectively in microliter volumes as in liter-scale reactions. 

One lysate, any scale – just add DNA


ALiCE® has been developed for optimal ease of use across scales. From microliter reactions in microtiter plates, through milliliter volumes utilizing conical flaks, to liter-scale reactions in bioreactors, our protocols are optimized for use with standardly available lab equipment. Whatever the scale, it’s the same simple protocol – just add the DNA sequence for your protein and let ALiCE® make your protein. Speak to our team to learn how ALiCE® can work with your established equipment.

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    One ALiCE lysate, multiple scales - microtiter plate reactions

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    One ALiCE lysate, multiple scales - mL in Erlenmeyer flasks

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    One ALiCE lysate, multiple scales - liter scale bioreactor reactions

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Interested in using ALiCE® for scaled protein production?

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A transferable process on track for GMP

 

We've made significant strides in expanding access to the power of ALiCE®, but our journey is far from over. Our objective is to ensure that ALiCE® is accessible for protein production at any scale, catering not only to research-grade proteins but also to clinical trials and biopharma manufacturing. With a robust ALiCE® manufacturing process in place, we're forging a clear path towards a fully transferable process and compliance with GMP standards.


Want to learn more? Reach out to us.

Are you interested in scaling your protein production with ALiCE®?

Our experts will be happy to answer your questions and discuss how you can access ALiCE® at scale.

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