Description
Features
- Protein production within 48 hours of DNA template addition
- Simple protocols using standard laboratory equipment
- Supports post-translational modifications and membrane proteins
- Suitable for scaled protein production
Description
ALiCE® for Scale-Up is a powerful protein production solution, optimized to support milligram scale protein expression for in-depth analysis and characterization studies.
Based on the same technology as our popular ALiCE® for Research Kits, ALiCE® for Scale-Up consistently produces high protein yields of over 2 mg/mL (eYFP standard). Originating from N. tabacum c.v. BY-2 cells, this eukaryotic cell-free expression system, contains native microsomes derived from endoplasmic reticulum and Golgi apparatus that allow easy production of proteins with membrane-spanning domains or eukaryotic post-translational modifications, including di-sulfide bonds and N-glycosylation. With everything required for protein expression included in the lysate (amino acids, energy supply, co-factors, etc.), the reaction is initiated by simply adding DNA.
ALiCE® for Scale-Up is provided together with two vectors, pALiCE01 and pALiCE02, which enable the user to produce proteins via the cytosolic or microsomal pathways for optimal flexibility. The pALiCE01 and pALiCE02 vectors are also hosted at 3rd party vendors (Twist and IDT), allowing simple synthesis direct of ready-to-use template DNA.
Note: ALiCE® for Scale-Up is also compatible with linear DNA formats. Details for optimized expression available upon request.
For larger-scale protein characterization and development projects, ALiCE® for Scale-Up provides the flexibility and scalability required to support your protein discovery objectives.
