A Powerful Expression System in the Hands of Experts
Our protein service employs a powerful eukaryotic expression system called ALiCE®. Harnessing the power of cell-free expression, ALiCE® has a strong track record producing varied protein classes, including those with complex features such as disordered regions, transmembrane domains and kringle structures.
Our team of protein scientists excels at maximizing the potential of our expression system from cloning strategy to scalable expression and purification. With ALiCE® as our cornerstone, we boast an exceptional expression success rate of nearly 100%, along with a proven history of overcoming expression challenges encountered in other systems.
Our Team Excel at Producing Challenging Proteins
We have produced varied proteins for our customers, many of them highly complex. These are just a few examples:
Targets
Membrane proteins and antigens, including a vaccine target with exceptionally low yields in other systems.
Complex enzymes
A therapeutic enzyme containing kringle domains and multiple glycosylation sites and disulfide bonds
Antibody variants
A varied panel of IgGs, scVs and Fabs expressed in a 96-well plate high throughput format.
We Offer a No-Cure-No-Pay Guarantee for our Feasibility Studies
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A Flexible Structure to Fit Your Needs
Your protein service project will start with a consultation led by one of our experts. Together, we’ll explore your specific protein needs and tailor the perfect solution for your project. Our modular packages feature clear deliverables and transparent pricing, allowing us to craft a customized package that aligns perfectly with your needs.
Every project begins with a feasibility study where we design optimized constructs and assess expression of your protein. We have such high confidence in our service that we offer a no-cure-no-pay guarantee for this crucial first step.
Once protein expression is established, your project gains momentum, with the first purified protein ready in as few as 10 working days from construct arrival.
Contact us for a call with one of our experts.
Cost-Efficient Scaling
ALiCE® eliminates the need for cell-line optimization for scaled production. Whether expressing simple or complex proteins requiring post-translational modifications or membrane integration, ALiCE® delivers consistent yields from 100 µl to 100 mL and beyond without requiring timely process optimization (Fig. 1 A and B).
With extensive front-loading during the proof of concept and small-scale protein production phases of a project, as you progress to larger-scale protein production with us, you can begin to realize the benefits of economies of scale. With minimal optimization needed for scaling ALiCE® reactions, costs remain manageable as your protein production grows.
Interested in Leveraging our Expression Expertise for your Protein?
Meet our Team
Our experienced in-house team of protein experts, with over 50 years of combined expertise, will handle your project from start to finish. Covering construct design and optimization, protein expression, extraction, and purification, we ensure meticulous care at every stage. With advanced techniques and attention to detail, we deliver exceptional results that exceed expectations. Trust us to guide your protein project with expertise and precision.
Maja Lieven
Head of Protein Service
Johannes Gottschalk
Senior Protein Production Scientist
Harendra Mahto
Analytical Scientist
Juliane Norf
Senior Protein Purification Scientist
Frank Albrecht
Senior Scientist DNA Solutions
Zulfaquar Ahmad Arfi
Alliance Manager for Protein Characterization & Analytics
Learn More About the ALiCE® Expression Technology
References
Das Gupta et. al., . 2023, J. Biotech. Bioeng.: https://doi.org/10.1002/bit.28461